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Questions related from David Scherf
Can anyone recommend a "good" general phospho-serine and/or phospho-threonine antibody for use after immunoprecipitation in a western blot? There are a lot of expensive offers online with...
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Could you recommend a good alpha or beta tubulin antibody that works nicely for yeast? I screened some publications and most of them use a hybridoma line antibody. However I would like to avoid...
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Which imidazole concentration do you use for protein- NiNTA elution? We use 300 mM, but i have seen protocols using 500 mM. Wouldn´t it be better, in theory, to use higher (>1/2 M)...
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