Next-Generation Sequencing (NGS) has become an essential tool in modern life-science research, generating vast amounts of data that provide insights into genetic variation, gene expression, epigenetic modifications, and much more. However, analyzing NGS data can be challenging, especially for those new to the field. This article aims to provide an overview of relevant topics for those unsure of how to begin with NGS data analysis: https://www.ecseq.com/support/ngs/getting-started-with-ngs-data-analysis-overview