Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

I was running Gaussian 09W Programmer in Frequency analysis but Chk File is not opened ?

gaussian frequency analysis was completed but chk file not open, Alpha MO energies Missing in view of the display.

24 January 2021 1,529 2 View

How to obtain the mobility from the EIS data?

Electrochemical impedance spectroscopy

09 August 2020 7,608 2 View

The resistance of the sensor was decreased after doping at room temperature why?

the resistance of the sensor was decreased after doping at room temperature why

18 June 2020 5,636 2 View

Hi, I would like to sense the Fe(III) in water, therefore how can I overcome the pH interference?

when adding the Fe compound in the water it gets deprotonated and from strong acid. 1.adding base leads to salt formation 2.adding buffer solution leads to Fe concentration change.

11 March 2020 1,785 3 View

Can anyone please suggest some curent research topics in Concrete Technology ?

concrete technology, materials

10 November 2019 8,423 12 View

Whether soil inorganic carbon sequestration leads to land degradation?

Dear senoirs friends, We know that carbon sequestrated as soil organic and inorganic . Several fancy literature reported that IC is high than OC. The organic forms usually labile and available...

22 March 2019 5,606 3 View

Can I use Laser Displacement Sensor (LDS) to measure strain?

I am building a small scale UTM machine. I have placed a load cell to measure stress. Can I use a LDS(Laser Distance Sensor) to measure displacement which can be ultimately used to measure strain?

22 April 2018 3,192 1 View

Whether non-significant data represented in chart or graphical representation?

22 September 2017 6,417 5 View

How much time will take to get first reviews from the journal DIGITAL INVESTIGATION?

How much time will take to get first reviews from the journal DIGITAL INVESTIGATION? Expect Reply from the paper those who submitted manuscripts already in this Jurnal. Thanks in advance.

16 September 2017 3,308 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View