How do you interpret and understand 11B 3Q MAS NMR graph?

I am a novice to NMR and need help in understanding what i am looking at in terms of 11B 3Q MAS NMR graphs. Any resources for beginners would be helpful in interpreting the data. Literature only...

20 January 2021 2,648 3 View

Is there a way to distinguish between overlapping energies in XAS graphs from the P L1-edge from the B K-edge?

Is there a post analytical methodology or experimental technique used to distinguish between overlapping energy edges in XAS?

09 September 2020 2,894 4 View

How do you apply chemometrics to Raman spectroscopy of glass samples with varying degrees of crystallinity?

What are the formula's and peak fitting types needed e.g. gaussian, lorenzian...

28 April 2020 2,301 3 View

Is there any other analysis methods one can do to analyse Raman spectroscopy data, other than stacking the plots and doing difference curves?

I am looking at glasses and the effect a certain component has on the peak positions and intensities. I do not have any complimentary techniques as of yet to help me analyse the data such as NMR.

27 April 2020 256 5 View

How to interpret and analyse data from accelerometer and gyroscope?

Hello, I am a research student looking into behaviour of horses. I am trying to use IMU devices to detect movement in horse movement (step frequency, lying down, etc). I understand how the sensor...

07 August 2018 5,646 3 View

What is the ideal procedure for testing anti-tumor with low cost price? What can you suggest procedure for extracting polysaccharides?

Hi! I am Katrina Co from the Philippines! I am just a High School student, I have only a limited samples for testing and a low budget (maybe around 6000 pesos or around $120). I only have limited...

29 May 2018 8,311 3 View

Any ideas for a protocol or extraction kit for extracting DNA from tomato fruit for the study of bacterial microbiota using NGS?

I'm trying to extract DNA from tomato fruit in order to study the bacterial microbiota found inside the fruit using NGS. I have tried using a CTAB protocol, but the precipitate is highly charged...

20 April 2018 423 3 View

I am looking for the protocol for DNA extraction from prostate biopsies?

I am planning to use COBAS 4800 to test for HPV in prostate cancer. What will be the best sample type for DNA extraction and the best protocol?

20 November 2017 2,387 2 View

Can anyone suggest a psychometrically valid, reliable, and quantitative measure of "Intentionality" attributions?

20 May 2015 5,982 2 View

How to check if the primer can attach to another organism from the same family?

I am working with two types of viruses from the same family. I was provided with specifically designed primers to test if either of the viruses are present in the material. However, I ran a PCR...

02 May 2014 4,866 4 View

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02 March 2021 3,060 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View