Neuroleptics such as thioridazine inhibit efflux pumps of mdr cancer cells. Any member of ResearchGate interested in collaborations?

01 January 2013 0 1K Report

We have found that phenothiazines inhibit ABC transporters by denying hydrolysis of ATP-

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Citations descending and ascending order by RG?

It seems to me that it would be extremely useful for the RG members to be able to have their citations by descending and ascending order much like Scopus. Companies can use this added criterion...

01 February 2018 2,696 0 View

Should the identity of the reviewer be made in the title page of the publication?

Reviewers perform an important role that results in the improved quality of a publication. However, with the exception of a few proceedings and books, they are not identified in the peer reviewed...

06 July 2013 3,400 42 View

Will nano antibodies directed against exposed TolC antigens of the RND efflux pump system inhibit efflux pump activity responsible for mdr phenotypes?

RND efflux pumps of Gram-negative bacteria bestow an MDR phenotype. Because deletion of the AcrAB-TolC main efflux pump of E. coli promotes the over-expression of the AcrEF-TolC as well as 8...

05 June 2013 7,354 0 View

Are efflux pumps of bacteria the means by which all secretions from the bacterium take place?

The first response of bacteria to a noxious agent such as an antibiotic is the induced over expression of efflux pumps. Once the threat of the noxious agent passes, the level of efflux pump...

05 June 2013 9,244 5 View

Should doctoral students of biomedical areas form an international society that supports their needs?

Doctoral students have needs that are not readily addressed by their mentors (supervisors). What are your needs as a doctoral student in your country and would the creation of an international...

04 May 2013 6,708 4 View

Are there any cancer cell lines that express more than one efflux pump responsible for the MDR phenotype of the cell?

Normal cells human cell types express different ABC transporters that when over expressed in the cancer cell render the cancer cell with an MDR phenotype. However, the genome of the normal cell...

04 May 2013 1,516 8 View

Besides novobiocin and chlorpromazine, are there other compounds that cure bacteria of plasmids?

A specific concentration of chlorpromazine eliminates plasmids from bacteria as a consequence of inhibiting plasmid replication whereas replication of the bacterial genome is unaffected. The...

04 May 2013 1,028 2 View

Can specific lasers matched to the energy level of a molecule create new molecules not readily made by conventional organic chemistry methods?

We have exposed a number of bioactive pure compounds to lasers of a given nm wavelength and energy level for varying periods of time, followed the result of exposure via thin layer chromatography,...

04 May 2013 6,697 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View