Nephrin antibody for zebrafish?

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How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

What should I do in this situation?

Dear Team of Researchgate, I'm writing this message because I realized somone is not the writer of the article called Propuesta intercultural para la enseñanza de cursos de español como lengua...

15 March 2024 9,705 2 View

Proceso de enseñanza aprendizaje híbrido de la física?

Fundamentar conceptos sobre este modelo de enseñanza aprendizaje de carácter híbrido aplicado a la física experimental

12 July 2023 2,138 4 View

Is it possible to use a single image to differentiate a SEM secundary electron image from a TEM image of a freeze-fracture replication of a sample?

I was challenged to identificate what kind of microscopy technique was used in five different images. The only information provided was the image, without other information, including scale bars...

14 March 2023 2,965 1 View

What are the risks of sharenting?

More and more adults are sharing content and photos of their children on social networks, but will this have negative consequences? It's a fashion? 📷

02 March 2023 2,473 2 View

How to measure short chain fatty acids by UPLC-MS/MS?

Dear researchers, We are starting a new experiment involving short chain fatty acids and we wanted to measure them in serum samples using UPLC-MS/MS. For the protocol set up, we found some...

03 April 2022 9,788 3 View

Why does my phage does not form proper bands in gel electrophoresis?

Hello, I have isolated a phage from a soil sample. I got a LTL somewhere around 10^9 pfu/mL. I did DNA extractions and got great DNA concentrations, always over 100ng/uL and the 260/280 ratio...

04 September 2020 6,078 3 View

How can I calculate the total average (or median) if I have 10 samples averages with different sizes?

I want to do some bibliography research and I have a table with the average age of 10 samples. Each sample has different size. Also I don't have the SDfor every dataset info.

07 January 2020 2,069 3 View

How long after my last allergen challenge can I measure Mouse Mast cell protease-1 (mMCP-1)?

Hi: I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues...

15 October 2019 9,532 2 View

What dilution should I use for my mice lung homogenates to perform a BCA total protein?

Hi community: I was wondering if you could give me more tips on the following. I am looking at cytokines levels of lungs exposed to an allergen. Specifically, I am looking at intracellular...

25 September 2019 6,887 7 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View