Nephrin antibody for zebrafish?

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How can I calculate the total average (or median) if I have 10 samples averages with different sizes?

I want to do some bibliography research and I have a table with the average age of 10 samples. Each sample has different size. Also I don't have the SDfor every dataset info.

07 January 2020 1,852 3 View

How long after my last allergen challenge can I measure Mouse Mast cell protease-1 (mMCP-1)?

Hi: I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues...

15 October 2019 9,291 2 View

What dilution should I use for my mice lung homogenates to perform a BCA total protein?

Hi community: I was wondering if you could give me more tips on the following. I am looking at cytokines levels of lungs exposed to an allergen. Specifically, I am looking at intracellular...

25 September 2019 6,686 7 View

Nephrin antibody for zebrafish?

I'm looking for a nephrin antibody with reactivity in zebrafish (western blot and immunofluorescence application). Thanks for help

30 January 2019 1,824 2 View

Normal epithelial ovarian cell line?

We are looking for a non-transformed immortalised ovarian cell line to use as a control in a dose-response experiment. We have found this line T1074 in abmgood. Do someone have this cell...

07 May 2017 3,065 2 View

How long do europeans spend to search a free parking lot?

I would like to know if there is any study that estimates how many minutes europeans spend to search a free parking lot per trip, for different urban zones.

12 May 2016 2,795 3 View

During an MD simulation the disulfide bonds are increasing over time, can someone help us with this?

We are doing a molecular dynamic simulation with GROMACS using the AMBER force field. During the simulation the disulfide bonds are increasing over time. We set the bridge manually with pdb2gmx...

14 June 2015 6,108 3 View

What is the best tool to obtain information abot unannotated genes?

I have information about N. crassa genome reference, but I need information about some genes with 'hypothetical protein' annotation. I have tried to find information in Uniprot, Blast, Embl and...

04 May 2015 1,859 4 View

Can I use SPSS to perform a CART analysis?

I wonder if it is possible to use SPSS to perform classification and regression trees, with the objective of performing a subtyping analysis. If this is the case, which command do I have to...

02 March 2015 3,139 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View