Hi:
I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues were collected 48 hours after the mice received the last challenge with HDM(house dust mite allergen) intranasally. I can't find in the literature the accuracy on the half-life time of this protease in the lungs.
Does anyone know if 48 hours is too late to try to measure this protein?
Also, if you have recommendations of what type of assay to use to measure this protease, I would truly appreciate it.
I am by no means an expert in this area but I can point you to a publication that may help. see attached publication
“The fact that 8% of the protease was still present in serum after 24 h indicates that the protease (mMCP-1) has a long serum half-life (at least 10 h).”
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays