Hi there everyone,

I have done immunohisto-staining for more than once now. I even used a tissue that I was positive that it will give results using B220 antibody. I tried changing the buffer from Citrate to TRIS/EDTA in order to see if the pH has anything to do with masking the antigenes. But still no results.. I don't know what went wrong! even thought I am really carful to do every step at it's specific time..Any one has any other trouble shooting suggestions that could help ..

Thank you so much in advance for answering