Amorphous XRD Data?

Dear colleagues, I analyzed my material using XRD analysis and the material has shown amorphous behavior , how can I specify the composition of the material is there any extra analysis I can...

24 December 2020 1,280 10 View

Can you put cells from -80 to liquid nitrogen?

Hi all, I have kept cells in -80 for several months, can I transfer them to liquid nitrogen now after all this time? I am afraid they would not survive.. I appreciate your input Thanks!

12 July 2020 3,612 2 View

Solar cell exciton binding energy ?

30 April 2020 1,620 12 View

How can you prevent cells from clumping on a coverslip of a 24 well plate?

Hi, I'm trying to stain my cells after transient transfecting them but each time I get the cells either over confluent, or clumped in the middle even though I swirl the dish in an 8 shape to...

15 March 2019 6,585 2 View

What is best ISI journal for "use game theory in human computation system and security" issue to submit article?

I want to submit an article in this area. please help me to find a good journal. thanks.

07 March 2019 1,694 3 View

Relationship between signal detection theory and drift-diffusion model?

When calculate signal detection metrics (e.g. sensitivity) from 2-alternative false choice task behavior data, we assume that the subject is internally comparing a variable to a threshold: if it...

03 March 2019 2,332 1 View

How to set and take beautiful images on a confocal microscope?

Hi everyone, I am newbie on using the confocal and I am wondering if the setting on all the images I take should be the same? in order to compare between them? because when I took the images I...

22 February 2019 1,815 3 View

What is the best algorithm for video to static image registration?

We have an wearable eye-tracking glasses that is video-recording the stimulus presented in front of the subject, and measuring gaze position in the coordinates of the video. However, we need to...

26 January 2018 3,278 4 View

AKT phosphorylation?

hi everyone, I have ben treating cells with a drug for 4 months now and then immunoblotted with AKT as it has been suggested that that drug effects the PI3k pathway. however, the phosphorylation...

30 June 2017 9,425 2 View

Sequencing analysis bascics findings

Hello everyone, I have done DNA sequencing on mouse samples after treatment with drug.. i wanted to see if there was any mutations caused by that drug.. I have found some mutations but...

29 June 2017 4,888 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View