14 Questions 5 Answers 0 Followers
Questions related from Delilah MH
Hi all, I have kept cells in -80 for several months, can I transfer them to liquid nitrogen now after all this time? I am afraid they would not survive.. I appreciate your input Thanks!
13 July 2020 3,821 2 View
Hi, I have been culturing 16HBE cells in MEM/10%FBS and suddenly I have lost the expression of E-cadherin in Western blot for these cells, even though they are normal epithelial cells and they...
05 July 2019 4,531 0 View
Hi, I'm trying to stain my cells after transient transfecting them but each time I get the cells either over confluent, or clumped in the middle even though I swirl the dish in an 8 shape to...
16 March 2019 6,715 2 View
Hi everyone, I am newbie on using the confocal and I am wondering if the setting on all the images I take should be the same? in order to compare between them? because when I took the images I...
23 February 2019 1,940 3 View
Hi everyone, so i have treated a cell line (that had KRAS ontogenically activated) with a drug that was thought to inhibit AKT phosphorylation. Indeed, Akt phosphorylation for the treated cells...
03 July 2017 7,327 3 View
hi everyone, I have ben treating cells with a drug for 4 months now and then immunoblotted with AKT as it has been suggested that that drug effects the PI3k pathway. however, the phosphorylation...
01 July 2017 9,559 2 View
Hello everyone, I have done DNA sequencing on mouse samples after treatment with drug.. i wanted to see if there was any mutations caused by that drug.. I have found some mutations but...
30 June 2017 5,100 3 View
Hello everyone, so I have blocked my membrane in 5% BSA then incubated over night with primary antibody in TBST then secondary in 3% milk. when developing I saw the band I'm looking for....
13 June 2017 2,140 4 View
Hi everyone, I'm planning to sequence my PCR product but first i want to make sure if i prepared my sample to be amplified by PCR correctly. I have added 10 μl 2x buffer (my taq ready mix) 1μl...
03 May 2017 4,300 5 View
Dear all, I am giving this drug which is 16.5mM in DMSO and I want to make 0.1mM and 0.33mM of it to add it to the cancer cell line. I know DMSO might have an effect on the cells so I'm planning...
02 March 2017 8,662 3 View
Hi everyone, What is better from your experience, counting cells manually or using automated cell counter? Thank you in advance
12 February 2017 2,101 5 View
Hi everyone, I accidentally left the antibody on the bench for one hour. is that going to effect its binding ability? Thank you in advance for answering :)
26 January 2017 9,098 10 View
Hi there, I am doing immunohisto-staining and all my results came negative.. I washed the sections with 200 microliter of PBS. and now I am thinking that I should have immerse them in PBS rather...
18 January 2017 7,327 8 View
I used antibodies to detect Ki67 in colorectal cancer and in normal tissue In the normal colon the Ki67 is found at the bottom of the crypt... and H3 is found in some mitotic cells. However, in...
12 November 2016 5,568 1 View
