Need of Agrobacterium AGL-1 strain with pBHt2 vector for Colletotrichum lindemuthianum transformation?

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Comment ajouter une recherche ?

En suivant toutes les étapes mentionnées sur (ajouter une recherche) il me signale erreur, ressayer, j'ai essayé plusieurs fois, sans succès, toujours il me signal erreur, ressayer

02 August 2024 9,719 1 View

Why practical assessments of dislocation density by XRD ignores the texture which apparently affects the result?

As one can read in numerous papers on this matter, the dislocation density is derived from the dependence of peak broadening on the diffraction angle, that is, on considered reflections. Thus, to...

28 July 2024 6,496 5 View

Is there any other efficient technique, as an alternative to multiple linear regression ?

with secondary data collected from central bank to know the impact of digitalization in banks profitability, how can its impact be more precisely measured through econometric tools.

21 July 2024 7,295 10 View

Hellow every one i have a question, please help me. How ESP car working?

I try to understand the ESP mechanism working. What kind of sensors it has, Is it necessary to modify or boost its mechanical ?

15 July 2024 4,772 1 View

In kalman filter, it is logical to have the observation variance equale 0 ?

I am working on implementing a Kalman filter integrated with ARMA parameters, as described in the article "Predicting Time Series Using an Automatic New Algorithm of the Kalman Filter"...

12 July 2024 8,116 2 View

Are diatom inclusions found in shellfish shells?

Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...

12 July 2024 159 2 View

How can I verify if an academic journal is a legitimate publication or a cloned version?

This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...

10 July 2024 5,966 2 View

Is there any guidelines about zooming out a chromatogram?

Hi, Just curious to know that in chromatography, a chromatogram should be zoomed to what scale? 10 times of output , 50X or ..? Is there any regulation or general chapter which describes this?...

03 July 2024 3,119 6 View

How to see whether my protein is aggregated using western blotting?

I am working on one protein which gets aggregated (similar to alpha-synuclein and tau) in certain neurodegenerative diseases. I am trying to confirm the aggregation using Western blotting....

30 June 2024 5,060 3 View

Do body proportions of sharks change after drying?

Dear colleagues, I would be grateful to anyone who can answer my two questions: 1. Do the proportions of the shark's body change after drying? In other words, is it possible to discriminate two...

30 June 2024 6,101 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View