Bilal A. Padder

10 Questions 73 Answers 0 Followers

Questions related from Bilal A. Padder

How much genome coverage is needed to perform QTLSeq?

I am working on common beans and a RIL population. I want to map QTLs via QTLSeq. To what extent I must sequence parents and bulks. I mean genome coverage from 50x or 100x or more than that

20 June 2019 3,060 0 View

SNP Bean Chip avalibility?

Can someone help me in finding BARCBean6K_3 beadchip which i want to use for fine mapping of bean anthracnose resistance loci. The help shall be highly

27 October 2016 5,245 1 View

Need of Agrobacterium AGL-1 strain with pBHt2 vector for Colletotrichum lindemuthianum transformation?

I want to start fungal transformation for identification of pathogenicity genes in colltotrichum. for this I am in need of AGL-1 strain with pBHt2 vector. Is any one having it and could spare it...

27 January 2016 8,172 2 View

In population genetics of phytopathogenic fungi what are the factors affecting fixation index (Fst) values?

I am working on fungus which is asexually reproducing. I have three populations with 14, 28 and 38 isolates (samples). After analyzing the ISSR data set I found low values of pairwise Fst. Now my...

14 January 2015 5,469 4 View

Can anyone tell me the steps of JoinMap 3.0 for making a genetic map along with the precautions I should take?

I am having SNP data of RIL populations and the Phenotypic data. Now I want to construct the linkage map using JoinMap. As I have no expertise of join map it would be nice if someone could provide...

09 December 2014 3,245 8 View

Can plant pathogenic fungi be stored for longer duration in a deep freeze conditions?

I have cultures of plant pathogenic fungus on Potato Dextrose Agar (PDA) medium. I usually store them in refrigerator and Paper Discs. However after repeated sub culturing they lose their...

23 August 2014 7,054 8 View

How much RAM should I use for assembling the data?

I have two data sets of NGS RNA Seq. I have a computer (Linux based with all the programmes of Galaxy) but with 16 GB RAM. When I start computing the de novo assembly, the computer does it for few...

28 May 2014 167 3 View

Can someone suggest where to find the following paper?

I am in need of manuscript titled Selection of a new set of homogeneously reacting bean (Phaseolus vulgaris) differentials to differentiate races of Colletotrichum lindemuthianum by Drijfhout and...

22 January 2014 9,509 4 View

Can anyone suggest how to do standardization of EST SSR's?

I designed SSR markers using MISA tool from the EST database downloaded from NCBI. The product size of one of the markers which I amplified in the PCR shows no specific binding. I also did...

05 November 2013 3,272 1 View

Adding scientists to a paper because the added author will pay APC charges

Nowadays many scientists add a few scientists for paying APC charges. The work described in paper has no relationship with the scientists who pays APC. The corresponding author acknowledge the...

01 January 1970 739 14 View