can any body know how to identify bacterial endophyte by SEM.
when we study presence of endophyte in plant sample (root and leaves) by SEM we observed different structure some are spherical, rod shape and several other structure.
how can we so sure the structure we are seeing are actually a bacteria.
kindly give me some paper, book and your suggestion.
Hi
Maybe these papers could be helpful:
Benhamou, N., Kloepper, J. W., Quadt-Hallman, A., & Tuzun, S. (1996). Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria.
Jacobs, M. J., Bugbee, W. M., & Gabrielson, D. A. (1985). Enumeration, location, and characterization of endophytic bacteria within sugar beet roots. Canadian Journal of Botany, 63(7), 1262-1265.
Quadt-Hallmann, A., Hallmann, J., & Kloepper, J. W. (1997). Bacterial endophytes in cotton: location and interaction with other plant-associated bacteria. Canadian Journal of Microbiology, 43(3), 254-259.
Good Luck
Please see:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990532/
http://aobpla.oxfordjournals.org/content/5/plt011.full
http://www.fungaldiversity.org/fdp/sfdp/33-4.pdf
Thank you all for the papers and i will discuss with you about these paper.
I read these paper. in one paper they use die to look endophyte, in one paper author study fungi in xylem.
but in SEM, size and shape is the criteria to say it is a bacteria.
i am attaching SEM images of green gram kindly help me are these bacteria or some other structure.
Gonzalo Galileo Rivas Platero, i have read the paper you gave me but i didn't understand the method of staining by Live/ Dead bacterial staining kit L13152. can i discuss this paper with you.
hope i will get your reply
http://aobpla.oxfordjournals.org/content/5/plt011.full
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