I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all calculated conc. shows different values (df 50 - 89.5 ppm & df 20 - 104.4 ppm). So, does using different dilution factors affect negatively on the values or there are other factors affecting those values ?
thank you for your time
Hi.
If you can discard errors in the dilutions, then it seems like a matrix effect. You can detail the method you're using or try spiking the dilutions and correct the concentration with the recovery factors. Should help.
Cheers
Muhammad Anas Yuzairi Mohd Yusri The linearity of the standard curve is important. When diluting samples always dilute them so that the readings fall in the mid range of the curve.
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