Dear all,
Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion day.
Unfortunately this time My hybridoma cell line has come up with a problem for which I dont have enough cells to continue to fusion, for which I need more time to stabilize my cells.
What can be done? Is it okay to continue more immunizations?