Mohd Shadab

10 Questions 25 Answers 0 Followers

Questions related from Mohd Shadab

Composition of Intermembrane space between cell membrane and cell wall?

I have found many reports on composition of Intermembrane space of organelle like mitochondria, chloroplasts, nucleus etc. i m not able to get information about 1) composition of intermembrane...

02 December 2019 2,519 2 View

Can we know the active bacterial samples in a metagenome by sequencing 16Sr RNA? Also suggest some techniques to isolate maximum amounts of rRNA

I have recently come across some papers that suggest 16S RNA sequencing as a technique to know the active microbial population in metagenome and require some views about it. Also if this...

24 May 2017 8,854 3 View

Does presence of RNA in NGS sample hamper its sequencing ?

we are interested in sending seed samples for NGS analysis but we are facing some problems as RNA was not removed even after treatment with RNase. we have used high concentration of RNase...

24 May 2017 7,206 3 View

Problem in plant regeneration?

I am working on tobacco and tomato plant tissue culture. one variety of tobacco giving very good result in regeneration. other variety causing some problem when it start shoot regeneration. its...

12 March 2017 4,578 12 View

Why tobacco plant cell suspension is brown in color?

I am using tobacco callus for suspension culture in MS basal and its color is brown. I did not find any contamination in suspension when plated in nutrient agar. callus was friable and white in...

12 March 2017 7,635 7 View

Can anyone help me how to induce shoot from tobacco callus?

I am not getting shoot formation from callus. I have used 2-8mg/l BAP and ten times less concentration of IAA. I found transformation in 3-5% of calluses. Only size of the callus increases in 16...

28 December 2016 3,732 11 View

Problems in Tobacco pant regeneration from callus ?

We are trying to regenerate shoot from tobacco callus. for this purpose we have used different concentration of cytokinin. 1-8 mg/l BAp, kinetin, 1-8 mg/l BAP+kinetin and callus was put in 16...

08 November 2016 8,562 13 View

Why do I have to add beta-mercaptoethanol in lysis buffer ?

I am trying to extract DNA from different plant samples. From tomato and tobacco seeds my RNA was remover by CTAB method after grinding with liquid nitrogen. But from green gram seed i am facing...

24 July 2016 7,081 1 View

Need help in presence of endophyte by Scanning Electron Microscopy?

can any body know how to identify bacterial endophyte by SEM. when we study presence of endophyte in plant sample (root and leaves) by SEM we observed different structure some are spherical, rod...

16 May 2016 5,121 5 View

I am facing problem in in-vitro seed germination of tobacco seeds. Can anyone help?

Can any one help me solving this problem. I am facing problem of tobacco seed germination in MS,media 0.4% water agar and filter paper. first seeds was sterilized with 0.4% sodium hypochlorite...

14 March 2016 6,201 10 View