Can anyone suggest which is the protocol for DNA isolation of nucleo cytoplasmic large DNA viruses
Thank very much . . !
1. There is much greater concentration of K+ ions inside the cell than outside, why then is the resting membrane potential always negative? 2. If you poison a neuron with a metabolic inhibitor...
09 October 2015 4,314 2 View
Hello, I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
11 December 2014 903 4 View
Hello, I am getting nonspecific bands in HIF-1alpha blot. Can any one suggest why and how I can improve this? I am using the following conditions Cell: HCT116 Gel: 8% Blocking: 5% non fat milk...
10 November 2014 4,956 5 View
Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...
09 October 2014 4,860 7 View
Hi, I am purifying proteins for crystallization, but sometimes they precipitate and degrade. What are the tips and precautions I should take during purification and storage? Is there any...
09 October 2014 777 14 View
I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...
08 September 2014 8,253 4 View
Hello, I want to know that how to check sensitivity of PCR ?
08 September 2014 313 8 View
Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...
08 September 2014 4,881 4 View
I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?
02 March 2014 10,128 13 View
Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?
01 February 2014 8,805 46 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View