I have some queries about basics of neuroscience...?

09 October 2015 4,230 2 View

How can CoCl2 mimic hypoxia?

11 December 2014 807 4 View

Why am I getting a nonspecific HIF-1alpha blot?

10 November 2014 4,862 5 View

How do you induce Hypoxia in cancer cell lines?

Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...

09 October 2014 4,765 7 View

How do you protect proteins from precipitation and degradation?

09 October 2014 690 14 View

How to identify Drosophila Mutant...?

I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...

08 September 2014 8,103 4 View

How do you check Sensitivity of PCR ?

Hello, I want to know that how to check sensitivity of PCR ?

08 September 2014 193 8 View

Why are tumor suppressor proteins down regulated in cancer cells?

Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...

08 September 2014 4,767 4 View

Can anyone provide me a protocol for Fusarium oxysporum culture?

I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?

02 March 2014 9,951 13 View

Does quantum mechanics fit to explain such brain functions as behaviour, neurodegeneration and consciousness?

Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?

01 February 2014 8,695 46 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View