I have some queries about basics of neuroscience...?

09 October 2015 4,230 2 View

Why am I getting a nonspecific HIF-1alpha blot?

10 November 2014 4,862 5 View

How do you induce Hypoxia in cancer cell lines?

Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...

09 October 2014 4,765 7 View

How do you protect proteins from precipitation and degradation?

09 October 2014 690 14 View

How to identify Drosophila Mutant...?

I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...

08 September 2014 8,103 4 View

How do you check Sensitivity of PCR ?

Hello, I want to know that how to check sensitivity of PCR ?

08 September 2014 193 8 View

Why are tumor suppressor proteins down regulated in cancer cells?

Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...

08 September 2014 4,767 4 View

Can anyone provide me a protocol for Fusarium oxysporum culture?

I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?

02 March 2014 9,951 13 View

Does quantum mechanics fit to explain such brain functions as behaviour, neurodegeneration and consciousness?

Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?

01 February 2014 8,695 46 View

How do you read and review a research article?

Anyone can suggest that how to read research article and review with proper understanding, because whenever I am reading articles I do not understand properly. Should I read some related...

31 December 2013 9,948 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View