I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?
@ Cynthia
Thank you cynthia.
Actually I want to isolate Fusarium oxysporum from Catharanthus roseus leaves. So can you please provide me a protocol for isolation and identification from Culture.
As you want to isolate F. oxysporum from plan parts, it could be useful to use a semiselective medium, such as the Komada medium (Komada 1975 Rev. Plant Protec. Res. 8:114-125). You can find the recipe at
https://sites.google.com/site/mediumrecipesexchange/fusarium
but it would be better if you can find the original article. Good luck
Thank you Giovanni. Now i am trying PDA medium. If i'll not gate from this i'll try Komada medium.
Thank you again.
Fusarium oxysporum generally exhibits a rapid growth in PDA/PDB medium, I would suggest you to maintain the stock culture in PDA plates, while you can have the working cultures in PDB broth
For isolation, identification and maintaining of Fusarium oxysporum we are using Czapek-Dox Agar media.
Czapek-Dox and Komada are both useful media for isolation of Fusarium.
What we also use with success is 1\2 strenght PDA with Streptomycin and Penicillin. Surface sterilisation of the tissue with 70% ethanol and rinse with sterile water afterwards.
As soon as you see hyphae grow out of the leave tissue transfer it to a new PDA plate. And of course you have to check if you picked up a pathogenic strain by infecting your plants with your new strain.
We use a concentration of 200ppm Streptomycin and 100ppm Penicillin.
Hi Manish,
I would also recommend the Czapek-Dox or Komada medium for Fusarium oxysporum isolation. If you already have the stock cultures and just want to grow them, use half strength PDA. Full strength PDA is a highly rich medium and your cultures will grow very fast.
One last piece of advice also, always inoculate F. oxysporum form stock, especially if you're using PDA, since it is a rich medium and consecutive cultures can easily result in mutations and loss of pathogenicity genes, resulting in a culture that's genetically different than your original stock.
Best of luck,
Vasileios
Any fungal media especially PDA is good for culturing F Oxysporum.
I face the same problem in growing fusarium oxysporum >> after one year of continuous sub- culturing on PDA medium the growth became transparent without apparent mycelium or spores . would you please tell me any solution for this case
thanks
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