ncer cell line? - FAQS.TIPS

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Whats the efficient way for the extraction of antioxidant peptide?

whats the efficient way for the extraction of antioxidant peptide?

03 April 2018 8,105 3 View

How natural compounds isolated from plants can contribute as soon as possible to concrete drugs?

02 March 2018 958 1 View

How can identify bioactive-comPounds from Plant samPles using innovative tools?

02 March 2018 2,905 1 View

Identification of compounds isolated from plant samples?

Dear colleagues, can you help me to get up the chemical class of natural-compounds which absorb at 305nm if purified by HPLC?

01 February 2016 2,717 3 View

Can anyone help with purification of natural product by HPLC?

Dear collegue To separate molecules from plant extract , we developped HPLC method , but peaks seem to be very close ! how to proceed to recuperate at least one of them ?

11 December 2015 1,112 5 View

Are you interested to study inhibitory effect of natural product on cancer cell line?

Are you interested ed to study inhibitory effect of natural product on cancer cell line?

01 January 1970 8,396 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Anca Onaciu

You can try to study the effect of a natural compound in more cancer cell lines and to compare the results. Also, you can look for some studies where your natural product was used to have some ideas of how it works on cancer cells

Salwa Bouabdallah

I have already developed this work and publish, but now I want to dig

enough benefits!

Many thanks