Whats the efficient way for the extraction of antioxidant peptide?

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How natural compounds isolated from plants can contribute as soon as possible to concrete drugs?

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How can identify bioactive-comPounds from Plant samPles using innovative tools?

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Identification of compounds isolated from plant samples?

Dear colleagues, can you help me to get up the chemical class of natural-compounds which absorb at 305nm if purified by HPLC?

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Can anyone help with purification of natural product by HPLC?

Dear collegue To separate molecules from plant extract , we developped HPLC method , but peaks seem to be very close ! how to proceed to recuperate at least one of them ?

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Are you interested to study inhibitory effect of natural product on cancer cell line?

Are you interested ed to study inhibitory effect of natural product on cancer cell line?

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ncer cell line?

ed to study inhibitory effect of natural product on cancer cell line?

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

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Mushtaq Ahmad

https://www.researchgate.net/post/Suggestion_for_extraction_methodology_isolation_of_peptide_seeds

may be useul

Emmanuel Ifeanyi Obeagu

Hi, You can use enzymatic hydrolysis in the extraction of antioxidant peptide.

Yumiko Yamashita

Article A Review of Antioxidant Peptides Derived from Meat Muscle an...

If you are going to extract peptides from animal tissue, this will be useful.