Dear collegue
To separate molecules from plant extract , we developped HPLC method , but peaks seem to be very close ! how to proceed to recuperate at least one of them ?
in start first select three or four peaks to extract out from hplc, but first figure out which one you need. but first if your peaks are very close then first you have to open them, if it is possible can I see your HPLC result , send me personaly your result then i can further guide you what to do.
Hi,
give us some more details. Are you interested in puryfing just one of the compounds or do you want to do something different. How is the sample extracted? Amount? Chromatograohic conditions? Do you fraction it? Can you use an additional / orthogonal purification technique e. g. preperative TLC?
All the best,
Holger
Thinks, i'm interested to purify the high peak area (5537 mau) ! about extract : it has been fractionned !but i will use usually HPLC , it's the diponible method in our laboratory!
if you are interested in my result ,i can send you the profile of my chromatogramme!
What Eluent you used to dissolve your sample? why your graph reading is going in - reading? like -500? and why your time is for 25 minutes? i think at least minimum you should set your time till 35 minutes.
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