Name of some mastermixes which contains UNG enzyme?

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In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it OK??

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it ok??

25 January 2021 5,336 10 View

Are two text files obtained separately from qiime 2 can be used together to make diversity graphs?

I have two sets of 16s microbiome sequencing data. The animal model, facility, diet vendor are the same in both sets. The only change is in the treatment. For analysis, can I use both the...

29 December 2020 6,976 3 View

How to estimate water volume using remote sensing?

I want to estimate water volume from different water surfaces. I have used Landsat data before. Is there any method to extract water volume from NDWI or other Landsat bands? If not then, what are...

21 October 2020 4,775 11 View

Any data available on traffic distribution for underwater wireless communication/sensor networks?

I am looking for any source of information that describes how often an underwater wireless communication network is utilised on a certain day. For example, if you consider an underwater wireless...

07 October 2020 4,340 6 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

Could you please recommend any good tutorials for natural ventilation in ansys with ref./pdf?

I am looking for a good tutorial for natural ventilation of a small room in Ansys fluent with ref/pdf for a detailed study.

22 June 2020 4,992 4 View

How to prepare 2x one step RT PCR mastermix?

Hello, I am working on developing a real time RT PCR based kit. For that, I need to prepare 2x one step RT PCR mastermix. I am using PCR Reagents and Verso cDNA synthesis kit from thermo...

03 May 2020 8,796 3 View

What kind of microstructure of Low Carbon Steel favors the maximum yield stress (>400 MPa)?

Micro-structural effect to Yield stress and yield ratio of low carbon steel.

13 March 2020 4,018 2 View

Why discontinuous yielding occurs?

The relation of discontinuous yielding with micro-structure of low plain Carbon steel

13 March 2020 527 4 View

Is it possible to increase the yield point of a Low Carbon(0.22%) Low Alloy steel from 320 MPa to 400 MPa by hot working? How or Why?

Low carbon low allow steel usually show low yield ratio. I want to investigate the reason behind this.

11 March 2020 7,035 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View