Hello,
i am new to the field of studying Reactive oxygen species in cells. I am trying to reduce ROS being produced in cells. I have tried L-NAC (N acetylcysteine), and I was wondering if there are any alternative drugs/compounds that I can add to cell culture to reduce ROS being produced in cells.
You can use Trolox, a vitamine E derivative. Remember, Vitamine E is a good anti oxidant.
Hello,
Depend if u are investigating general ROS or specific cellular sources.
You can try Tempol (similar of NAC) or Vitamin E for general ROS.
On the other hand, if u are interested in a specific target such:
1) Mitochondria: Mitotempo
2) NADPH oxidase: apocynin or GSK2795039
3) NO synthase: L-NAME
4) xantine oxidase: Allopurinol
There are others examples depending on the source or isoforms (e.g. NOX2 or 4).
Try to build a curve-dependend response to fix ur protocol before set your experiments.
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