How can i dissolve PALMITIC ACID?

26 September 2023 1 965 Report

Please i have been trying to dissolve Palmitic acid in BSA-NaOH, it keeps solidifying after heating. I wanted to used DMSO but its' lethal to cells. And also the DMSO recommended concentration to cell/medium should not exceed the range 0.1-0.5, and this concentration couldn't dissolve it.

PLEASE GUIDE ME THROUGH.

Thank you.

Malcolm Nobre

Dear Mansur Dabai Salisu

You may dissolve 51.2mg Palmitic acid (mol.wt. of Palmitic acid is 256.43 g/mol) in 1ml ethanol (100%) to give a stock of 200mM.

Take 40ul of the above stock solution and add to 1.96ml of 10% fatty acid free BSA (in culture media) to give 4mM diluted stock of Palmitic acid. The solution must be shaken overnight at room temperature.

The following day, you may filter sterilize the diluted stock using 0.22μM filter. If required, further dilutions to be used in cell treatment may be made in culture media.

Best.

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

More Mansur Dabai Salisu's questions See All

Can I perform a Benjamini Hochberg FDR correction over p values from different tests?

I'm performing statistical analysis on a time series data, comparing the average amplitude (y axis) between three groups on every 50 ms (x axis). The time series is 1200 ms long (so in total 24...

21 October 2020 3,269 3 View

Please, I need clarification on the quality status of a journal listed in WoS Zoological Record. Is this index comparable to SSI, ESCI, etc?

So many journals claim they are indexed in WoS (ISI in old parlance; now called Clarivate Analytics). However, most of the journal end up listed in the Zoological Record (ZR). Reading about that...

03 March 2020 1,733 3 View

Hello ! Is it possible to use genomic DNA(gDNA) to check gene expression instead of using cDNA ?

Actually I m doing gene expression study and thought that we can save our time to directly use gDNA to check gene expression instead of following long protocols of RNA isolation and cDNA making

01 April 2018 8,747 5 View

Which simulator is best suited for IoT security?

15 March 2018 1,644 3 View

Can you suggest any validated measure of trait emotional intelligence (trait EI) that has the fewest possible number of items?

The measure should preferably be domain-specific to teacher-leader trait EI.

03 April 2017 2,398 5 View

Why, when using FHI-aims, did I find series of frequencies (about 300 of them) with more than half of them negative?

11 June 2015 6,526 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

H2O2 scavenging activity problem??

When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...

09 February 2021 6,430 2 View