nothing
My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?
31 May 2024 454 2 View
what are the possible reason(s) that make AsPC-1, 293T and MCF-7 cell lines failed to be transfect/transduced?. Please help me with possible hints?
10 March 2024 8,812 2 View
I have calculated band structure of a halide perovskite consisting Flourine but its gap is lower than the perovskite with Cl how is this possible?
28 February 2024 1,527 1 View
Phonon dispersion problem related to half heusler compound
21 October 2023 4,928 3 View
Please i have been trying to dissolve Palmitic acid in BSA-NaOH, it keeps solidifying after heating. I wanted to used DMSO but its' lethal to cells. And also the DMSO recommended concentration to...
25 September 2023 990 1 View
If i want to calculate heat capacity for half Heusler compound is the heat capacity equation will be same i used in halaideperovskite compound in DFT?
18 September 2023 6,998 0 View
Half heusler alloy electronic property under pressure
12 August 2023 5,002 3 View
I'm performing statistical analysis on a time series data, comparing the average amplitude (y axis) between three groups on every 50 ms (x axis). The time series is 1200 ms long (so in total 24...
21 October 2020 3,568 3 View
Questionnaires and Checklists seem to be the most widely instruments used for data collection in survey studies among undergraduate Students.
12 June 2019 4,793 5 View
RQ1. To what extent, if any, is there a relationship between project manager’s cultural intelligence and IT project success? RQ2. To what extent, if any, is there a relationship between project...
29 May 2018 8,016 20 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...
02 July 2024 4,887 0 View
For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...
30 June 2024 2,891 1 View
