Geir Bjorklund
Poor quality or contaminated cell culture media: Using fresh, high-quality cell culture media is crucial for cell growth. Contaminants such as bacteria or fungi can cause cell death or slow growth. Inadequate conditions for cell growth: Cells may grow slowly or die if the temperature, humidity, or CO2 levels in the incubator are not optimized for their growth. Insufficient mycoplasma treatment: SiHa cells can be prone to mycoplasma contamination. If the mycoplasma treatment was not effective, the cells may continue to be affected, leading to slow growth and cell death. Overcrowding of cells: Overcrowding of cells in the culture dish can lead to a lack of nutrients and oxygen, resulting in slow growth and cell death. Aging of cells: Cells have a limited lifespan and can eventually become senescent, resulting in slower growth and death.
There could be several reasons for slow cell growth and cell death in your SiHa cells. Some possible explanations are:
It is important to carefully monitor your cell cultures and adjust conditions as needed to ensure optimal growth and minimize cell death. You may also consider using alternative cell lines or repeating the mycoplasma treatment to confirm that it was effective.
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