My sequence is not passing the validation when submitting to genbank, how can I overcome this?

22 February 2023 0 6K Report

Hello, I am completely new to Genbank submission. I am currently submitting a whole genome sequence however, my sequences does not pass the validation.

The message displayed is the following:

'Sequence is shorter than 200 nucleotides'

Please could you suggest what I can do to resolve this?

Badges
Science topic

More Alice Woodhouse's questions See All

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Once I annotate a genome with PROKKA, where do I uplaod it?

I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...

29 December 2020 3,976 3 View

How can I get Genbank accession code given the scientific names?

Hello! I now have a complete list of about 23k plant species that I want to check if Genbank has the sequencing data for a phylogenetic analysis later, and I am really new to the topic. Is...

02 November 2020 4,450 3 View

How to retrieve the lengths of all DNA-Sequences inside the NCBI GenBank?

I want to perform some statistics on the lengths of DNA-Sequences inside NCBI Genbank, for example, I want to draw a histogram of these lengths. So I am asking is there a way to retrieve the...

21 August 2020 8,531 3 View

Problems extracting metadata from NCBI in R?

I am trying to extract some information (metadata) from GenBank using the R package "rentrez" and the example I found here https://ajrominger.github.io/2018/05/21/gettingDNA.html. Specifically,...

12 August 2020 5,742 2 View

Why do the rentrez and NCBI web searches return different number of hits?

I am querying the GenBank and getting different numbers when compare searches in R (rentrez package) to the Nucleotide db at NCBI website. For example, entrez_search(db="nuccore", term =...

17 June 2020 7,127 3 View

Designing PCR primer for Sars-CoV mRNA ?

I need to design primers for PCR reaction for SARS-CoV mRNA strand...

01 June 2020 7,100 3 View

How do I batch download a set of GenBank files (in .gb format) into separate archives?

Hi! I need to create and maintain an updated database of all complete and annotated SARS-CoV-2 genomes that I could find at NBCI. I am pretty new to this, so I managed to get until the point...

13 March 2020 7,526 9 View

How to align multiple sequences (different genes) to a reference/query sequence (complete genome)?

I am working on FIV and have sequenced each gene individually and retrieved multiple complete genomes and individual genes from Genbank. I am trying to align all the different genes to the...

02 March 2020 1,419 1 View

How to make a GenBank or tab delimited format file from the output files of CPGAVAS? Which output file should be chosen for making GenBank ?

I have annotated chloroplast genome by using CPGAVAS web tool and got output files. further, i want to use OGDRAW to draw circular map but this requires GenBank format file. Please tell me which...

24 February 2020 3,605 3 View

Potential of miRNA and svRNA-N antagomirs as antivirals Missed in Chinese (ethnicity) This is engineered virus, Will stop after 35 days?

Potential of miRNA and svRNA-N antagomirs as antivirals Missed in Chinese (ethnicity) This is engineered virus, Will stop after 35 days. Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1,...

31 December 2019 6,363 2 View