Can I preserve my COMET assay slides?

01 August 2014 7,768 17 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

03 May 2014 9,979 5 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

11 April 2014 6,336 23 View

Does dead bacteria sink or float in its growth medium?

I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...

23 March 2014 3,276 7 View

What is the recommended incubation time for my cells to be treated with extract (for MTT assay)?

I need to pre-treat my cells with a plant extract first. What is the recommended incubation time for my cells to be exposed to my extract for this MTT assay- 24 hours or 48 hours? I'm using...

10 February 2014 3,379 5 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View