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Questions related from Jhoti Somanah
Hi, I'm doing the Alkaline COMET assay. But since I have many samples I wanted to know if it is possible to preserve my slides after doing the electrophoresis? I want to preserve the slides, so...
02 August 2014 7,821 17 View
These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...
04 May 2014 10,029 5 View
Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...
12 April 2014 6,785 23 View
I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...
24 March 2014 3,313 7 View
I'm using DMEM with glutamine and 10% FBS. Since a month now, the growth rate has seriously dropped. No contamination has been detected. What can I do to make them grow quicker?
18 February 2014 7,132 4 View
I need to pre-treat my cells with a plant extract first. What is the recommended incubation time for my cells to be exposed to my extract for this MTT assay- 24 hours or 48 hours? I'm using...
11 February 2014 3,434 5 View
Would you recommend treating cells with a plant extract for 24 hours or 48 hours?
11 February 2014 2,314 3 View
I'm frequently experiencing contamination of my cell cultures and strongly suspect it to be due to the water in my CO2 incubator's reservoir. The incubator is quite big, so it is difficult to...
06 February 2014 9,853 1 View
I'm experiencing frequent contamination of my cell cultures despite using fresh media (with antibacteria and antifungal agents added), new materials and improving my handling techniques. I...
06 February 2014 2,931 30 View