Jhoti Somanah

9 Questions 20 Answers 0 Followers

Questions related from Jhoti Somanah

Can I preserve my COMET assay slides?

Hi, I'm doing the Alkaline COMET assay. But since I have many samples I wanted to know if it is possible to preserve my slides after doing the electrophoresis? I want to preserve the slides, so...

02 August 2014 7,860 17 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

04 May 2014 10,066 5 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

12 April 2014 6,908 23 View

Does dead bacteria sink or float in its growth medium?

I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...

24 March 2014 3,352 7 View

My fibroblast cells are growing very slowly, what can I do to accelerate their growth?

I'm using DMEM with glutamine and 10% FBS. Since a month now, the growth rate has seriously dropped. No contamination has been detected. What can I do to make them grow quicker?

18 February 2014 7,175 4 View

What is the recommended incubation time for my cells to be treated with extract (for MTT assay)?

I need to pre-treat my cells with a plant extract first. What is the recommended incubation time for my cells to be exposed to my extract for this MTT assay- 24 hours or 48 hours? I'm using...

11 February 2014 3,473 5 View

For cytotoxicity testing, should I pre-treat my cells with extract for 24 hours or 48 hours?

Would you recommend treating cells with a plant extract for 24 hours or 48 hours?

11 February 2014 2,348 3 View

Is it possible to use an autoclavable dish filled with sterile water and CuSO4 in a CO2 incubator?

I'm frequently experiencing contamination of my cell cultures and strongly suspect it to be due to the water in my CO2 incubator's reservoir. The incubator is quite big, so it is difficult to...

06 February 2014 9,894 1 View

What's the best way to clean my CO2 incubator?

I'm experiencing frequent contamination of my cell cultures despite using fresh media (with antibacteria and antifungal agents added), new materials and improving my handling techniques. I...

06 February 2014 2,975 30 View