Dear All,

I am trying to standardize Acetic acid/ Urea PAGE electrophoresis for protamine separation. I am able to form stacking and resolving gel. But i am not able to detect any protein on the gel. Also, according to the protocol, it should be run in the reverse polarity. But when i am running the gel sample dye is not entering in the gel.

Please, anybody, suggest the changes.

Thank you in advance...!!