Riddhi Kirit Pandya

4 Questions 7 Answers 0 Followers

Questions related from Riddhi Kirit Pandya

Can anybody share the protocol for Acetic acid/Urea PAGE electrophoresis?

Dear All, I am trying to standardize Acetic acid/ Urea PAGE electrophoresis for protamine separation. I am able to form stacking and resolving gel. But i am not able to detect any protein on the...

10 October 2019 4,190 2 View

How much sequence coverage is needed for global DNA methylation analysis by bisulphite conversion and sequencing of LINE1 gene?

I am trying to understand the levels of methylation using LINE1 gene (surrogate marker for global methylation) in mice. My aim is to see the levels of methylation between control and test, hence i...

07 July 2019 2,761 3 View

How to separate low molecular wt protein on SDS-PAGE?

Molecular weight of my protein of interest is 7KDa. I am not able to get this on 18% SDS-PAGE electrophoresis. I want to do western blotting of my samples having this. Kindly suggest the method to...

03 March 2019 4,352 8 View

MspI/HpaII enzyme digestion can be used for checking global DNA methylation?

I want to see global DNA methylation status of samples. What are the different ways by which i can check this? After doing bisulphite conversion, what all genes i can sequence to understand level...

03 March 2019 7,755 4 View