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Questions related from Chitra Devi
In order to prepare conditioned media from primary cultured dental pulp cells, passage 5 is widely reported. May I have some scientific explanation despite of the inadequate quantity of the cell....
10 January 2024 965 2 View
I noticed layer was formed during NK cell culture (initial day) of a cancer patient. Could it be platelet aggregation? What are the causes of layer formation in NK cell culture?
24 November 2023 6,732 0 View
I started to use RossetteSep for my NK cell isolation with slight modification of manufacturer's instruction as follows:- Manufacturer's instruction: RossetteSep is added into the whole blood...
13 June 2023 2,286 0 View
There are numbers of studies showed SHED usage in neural degeneration. I need advice on the usage of dental pulp stem cell derived from permanent teeth for neural degeneration. My subject group...
09 January 2023 6,977 2 View
How to prepare cells derived from mesenchymal stem cell for flow cytometry? (1) Cells in PBS and stored in -80? (2) Cells in sterile saline and stored in -80? (3) Cells in PBS and stored in 4...
06 December 2022 416 0 View
My study showed an increased plasma Ang II level in WKY rats after high-salt intake. This is unusual in normotensive condition; any explanation?
10 September 2018 4,755 0 View
Could anyone suggest me any tools to identify gene of interest from transcriptome data?
05 July 2016 981 2 View
Anyone have tried QuantiTect Reverse Transcription Kit from Qiagen?
11 February 2016 5,103 4 View
Dear fellow researchers, I am working on rat brain tissues. I need to extract RNA from here. The recommended RNA concentration is >150ng/ul (reading from Qubit). I am using Qiagen products and...
02 October 2015 6,855 13 View
The determination of passage number (PN) is dependent on phenotypic characterisations. How do we really choose the correct PN especially for HUCMSC for therapeutic use?
01 January 1970 7,601 0 View
I am isolating NK cells from PBMNC. The yield of NK cells at the end of culture was found to be less than 60%. Kindly recommend me (1) The NK culture media that can activate and enhance expansion...
01 January 1970 8,282 0 View
