Hi everyone,
I have DNA templates for T7 IVT mRNA transcription that are between 250 and 1050 bp long. I want to run T7 IVT mRNA synthesis using NEB2080 kit as recommended, but since tempates are so small, would you recommend to run IVT incubation overnight? For how many hours?
Any other recommendations would be greatly appreciated.
Thanks!
Kind regards,
Maria