What is the maximum nontoxic concentration of doxycycline for human iPSC cells?

27 January 2022 1 1K Report

Dear colleagues,

I have applied 1 ug/mL of doxycyline to human iPSCs but most cells died within 24 hours after treatment. I was wondering if you have applied doxycyclin to iPSCs and know what concentration of doxycyclin is non-toxic for the iPSCs to still be able to differentiate?

Kind regards,

Maria

Shambhabi Chatterjee

Dear Maria Victorova

The highest doxycycline concentration I have tested is 4 uM/ml (for 3 weeks with fresh medium change everyday). The hiPSCs tolerated it quite well and were growing fine and differentiated at similar efficiency as the no doxycycline controls.

Perhaps reducing the concentration in your experimental setup might help.

Good luck!

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