I need to transfecte podocyte primary and/or cell line with lipofectamine 2000, I will be verry happy if someone can provide me a clear and detailed protocol.
Thank you so much for this nice protocol, I will try it and I will say you if it work in my condidion. Did you previously used it on primary podocyte or podocyte cell line? Have a good day!!
As you know, often times it is very difficult to achieve high transfection efficiency in primary cells. May I ask if you have tried Self-Deliverable RNA silencing FANA Molecules to achieve high delivery efficiency in primary cells?
If not, I encourage you to review the attached FANA delivery protocol without the use of any transfection reagents.
Please kindly let me know if this can help your specific experiments?