Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability?

I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...

24 September 2020 2,931 1 View

Article - Research to evaluate the compaction of soils under > 2 years test ?

13 August 2018 9,973 4 View

Mortality rate for BL6C57 8 weeks female mice in 3% DSS colitis model?

Hi all, I would like to know if someone has ever done DSS (3%) colitis in BL6C57 8-9 weeks old female mice? I am seeing quite variable results between different cohorts and in the last cohort I...

08 March 2018 9,171 4 View

Normality Test problematic: too low samples in ANOVA. Any solution?

Dear All, An experiment was carried out, using as factors the temperature, the humidity and in addition, it was carried out for 6 different species. The total sample number of species analyzed is...

13 December 2017 577 3 View

There are some matrix for encapsulation of bateria that allowe slow release?

I want to prove some matrix to do a biofertilizer that allow slow release of bacteria.

15 November 2017 8,389 4 View

I am having much lower current density in mp-TiO perovskite devices comparing with the Al2O3 made ? What can I do?

Hi I am fabricating mp-TiO2 Perovskite solar cells (Glass/ftO/bl-TiO(50 nm)/mp-TiO/CH3NH3PbI3-XClx/Spiro/Au), but I am getting low current density (10 mA cm-2), Voc is 0.74 V and FF is 0.55. I was...

16 May 2016 7,375 5 View

I am extracting DNA from a Gram(+), how to improve A260/A230 ratios?

22 February 2016 5,443 2 View

Does anyone know a suitable HPLC method to analyse organic acids such as formate and lactate as well as others SCFA?

We are trying to analyse organic acid production from bacteria supernatants by HPLC. We tried some methods that we found in the literature but we are having problems with some organic acids like...

03 April 2015 9,379 12 View

Can you provide information on Gonodonta pyrgo?

21 July 2014 230 3 View

Can you provide information on Gonodonta pyrgo?

06 July 2014 7,190 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View