Cristina Torres-Fuentes

6 Questions 27 Answers 0 Followers

Questions related from Cristina Torres-Fuentes

Mortality rate for BL6C57 8 weeks female mice in 3% DSS colitis model?

Hi all, I would like to know if someone has ever done DSS (3%) colitis in BL6C57 8-9 weeks old female mice? I am seeing quite variable results between different cohorts and in the last cohort I...

03 March 2018 2,080 5 View

Does anyone know a suitable HPLC method to analyse organic acids such as formate and lactate as well as others SCFA?

We are trying to analyse organic acid production from bacteria supernatants by HPLC. We tried some methods that we found in the literature but we are having problems with some organic acids like...

04 April 2015 9,434 12 View

Does anyone know some minimal media for growing Lactobacillus and Bifs?

I need to test their supernatant on my mammalian cell culture but it interferes with my assay. I need some media with really low Carbon source but still enough to allow the bacteria to survive...

04 April 2014 1,281 12 View

Does anyone have a suggestion for bacteria lysis by sonication?

I have to do some screenings with Lactobacillus and Bifs in mammalian cells. I will carry out the study of the cell lysate. I think, the most easy way is sonication. Which power/time/number of...

04 April 2014 6,464 13 View

Does anyone have any protocol regarding probiotics supernatants, microbial cell extract, and/or non-viable microorganisms preparation?

I need to study some beneficial effect of different potential probiotics strains on HEK cells. I would like to know the best way to prepare these probiotics samples to test them on mammalian cell...

03 March 2014 3,047 3 View

Can anyone help with bacteria and mammalian cells co-culture?

I have to study some effects of probiotic bacteria on HEK-cells. In particular, I have to analyse their modulation activity on a receptor which my cells are overexpressing. I have checked in the...

03 March 2014 1,577 27 View