How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability?

I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...

24 September 2020 2,931 1 View

Is there any tool to measure impact of climate change on wellbeing of people of a particular community? Please suggest?

Climate change is a topic that should be given so much importance. How can I go forward with this topic for studying the impact of climate change on people's well being?

14 September 2020 3,141 4 View

How can I measure the impact of climate change on the wellbeing in India?

I am very much curious to learn the impact of climate change on the psychological wellbeing or wellbeing of the person in general. Is there any standardized tools to find this out. How can I go...

29 July 2020 1,141 4 View

Cas9 in vitro clevage, no bands or bigger bands?

12 February 2020 8,781 1 View

How can I measure antioxidant capacity of cosmetic lotion?

Dear scientist: I want to measure the antioxidant capacity of cosmetic creams and lotions with the DPPH and ABTS methods, but I am not sure how to prepare the samples. Will dissolving them in a...

10 February 2020 3,597 4 View

Random sampling from a dataset, preserving original probability distribution, with R sample function?

Hi everyone, I have a question concerning random sampling from a dataset. I would like to extract random samples from a dataset. I thought the "sample" function in R could be a good and simple...

10 February 2020 8,061 2 View

Can you help me know if there is any provider of pressure based flow control system for microfluidic similar to OB1 MK3 of Elveflow in Mexico?

We are interested in developing a electrospinning system and need: Flow rate: 1-5 mL/h

04 December 2019 239 1 View

Discrete event pattern recognition in time series not equally distributed?

I have more than 400 different events that occur during two years, some of them can occur 4000 times an others no more than 50 times. These events are not equally distributed, e.g., one at...

20 November 2019 5,083 3 View

Can I envoy pure RNA with RNA stabilizer?

Has anyone ever envoy pure RNA with RNA stabilizer? How to sent (2 days away) pure RNA to RNA seq without dry ice? It is a good idea to pellet RNA and then submerge in RNA stabilizer? I have sent...

10 November 2019 3,152 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View