Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Why have a specified percentage absorbance to be reached for flow test on UV Vis?

When performing a flow test the objective is to find the total amount of blank to be replaced by sample with pump. Why would a specified percentage of 100% i.e. 99.5% of that absorbance be used?

17 December 2020 7,762 3 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,532 3 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

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08 January 2019 3,512 5 View

How do you construct a crRNA for pCas9 (addgene 42876)?

Hello, I'm designing a CRISPR/Cas9 protocol for school purposes (I attend a extra project next to my study). I have to use the pCas9 plasmid (addgene #42876) for CRISPR eventually. This plasmid...

08 July 2018 5,461 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

How to reflect human lifespan in rat model?

I am looking to model different ages (in humans) using a rat model. Does anyone have any papers which describe the developmental markers in brains of rats and humans? Example) How many months...

06 March 2018 5,880 4 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

At what Forchheimer number does flow (in a porous media) transition from Forchheimer like to fully turbulent? (Literature references appreciated)

22 May 2017 3,830 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View