MitoSpy vs. MitoTacker?

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How do the complex dynamics of the oral microbiome interact with the host immune system affect periodontal disease development and treatment response?

This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...

09 July 2024 7,922 1 View

What is the concentration in mL of glycerol and Equex Paste is required to dilute semen to a concentration of 1 x 10^6?

The semen concentration will then be diluted with fraction B (9% glycerol and 1.5% Equex Paste 13560-0008) at 50 °C to a final concentration of 1 x 106 spermatozoa/mL.

23 May 2024 9,071 0 View

Who has research on healing attachment wounds in male-identified populations?

I am interested in looking at male attachment, including the development of attachment wounds, systemic and cultural factors. I’d like to specifically tie together the potential impact of healing...

10 February 2024 656 2 View

What sort permits do I need to import eDNA samples into the U.S.A.?

I'm working with a student overseas who wants to bring environmental DNA (water filters preserved with ethanol) to my laboratory in the U.S. for a research project. It is not entirely clear to me...

03 December 2023 5,969 2 View

What is causing grid-like shadows on my stitched Keyence images?

I do not like how my Keyence BZ-X710 images have been coming out lately. I’ve been noticing grid-like shadows on the images after stitching them, but not while looking at the live images. The...

14 September 2023 7,394 1 View

Database with labelled CTs of brain?

Dear all, I'm currently looking for a dataset with labelled (unenhanced) CT brain images, with at least the ventricles and the brain matter segmented. I've already looked through the links on...

24 August 2023 5,612 1 View

How can a steel ball form reciprocating motion in the cage under lubrication conditions?

What additives or lubricants are required for the operation status of the steel ball in the fixed section of the CVJ of the car, or at what friction coefficient can the steel ball not move at a...

05 August 2023 2,934 1 View

Hey I have my gene of interest in a pacebac plasmid. the cells we have are DH10bacs and not multibacs. Can I go down this route? or should i order?

Normmally you would go from pFastBac to DH10Bac or paceBac to Multibac. I currently have paceBac and DH10Bac and want to avoid ordering multibac if possible. Thanks!

20 April 2023 6,329 2 View

Is it possible to add a covariate to a linear mixed model in SPSS?

I am currently working with a data set where I would like to answer the research question using a linear mixed models approach. I would like to introduce 2 categorical covariates into the model,...

11 October 2022 6,867 3 View

Mineral formula recalculation for F and Cl bearing silicates?

Hi All, I have only recently started including F and Cl in my microprobe analyses. I am curious how to calculate a mineral formula including F and Cl when assuming a number of oxygens. For...

08 March 2022 7,636 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

How can magnetic Biochar remove dye pollutants, is this technology beneficial?

The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

07 July 2024 8,118 1 View

Sybergreen qpcr troublshoulting?

500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...

06 July 2024 5,478 4 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View

Chathura S. Abeywickrama

Dear Jesse,

Please see following links:

https://www.biolegend.com/en-us/mitospy

https://www.thermofisher.com/order/catalog/product/M7512?SID=srch-srp-M7512#/M7512?SID=srch-srp-M7512

It looks like they are structurally same.

Thanks

Beatriz Martins

I work with Mitospy. If you want I can send you some pictures of the staining in BV-2 cells