Miseq with cluster density of 1150K/mm2, but passing filter failed. what is the reason?

01 August 2021 0 7K Report

I spiked my library with 10% PhiX, and loaeded on Miseq at 13pM, using V3 300 cycles kit. the cluster density of 1150K/mm2, but passing filter failed. I prepared a normalized pool again and loaded on a different MiSeq, and I got the same problem.

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