Hi everyone

I get some confused with the normalization of my library. I am going to sequenced with miseq system, i alredy have the library prepared, but i am not pooled it yet, because the samples have diferent nature, i am working with samples of 200, 700 and 900bp, i know that illumina work with molarity, so i have to normalize my samples before pooled then. i dont know if i have to put them in a standard concetration first (example: of 10 ng) and then normalize to molarity, or if i can normalice to molarity with the concentration wich my samples have without diluted them.

i'll really appreciate if someone could help me!