Hi all!
I have paired end reads of the V3-V4 region of the 16s. PCR amplification and sequencing was performed at macrogen facilities, in Korea. Sequencing was performe with Illumina MiSeq.
I have the following doubt:
Does macrogen removes primers from 16s sequences before delivering the final data?
Alternatively, how can I check if 16s sequences still have its primers?
Thanks in advance!
This is something which you can ask the company. But, you can also check it easily if you have some basic understanding of CLI or know how you can do it in any other way.
But with this question, it appears that you probably need to invest some time in learning the basics. If you are proceeding with the data analysis directly, it will not be easy and you will be facing other data related problems as well.
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