Hi, I am a novice in the field ..so I have doubts and looking for your expertise. I found few miRNAs in blood that found to be differential expressed in diseased patients. With computational approach I found certain targets for this miRNA. So I want to validate them. 1) HOW TO FIND THE GENE CORRESPONDING TO microRNA ? what should I consider mature sequences,pri or pre sequences? Any illustration or tool or video links? How to study the experimental validation? Possibles ways and pros and cons? Thank you very much,
Hi,
I think your interested in motifs that microRNA bind too.
You want too see if your microRNA motifs, respective to your microRNA can be found in your gene list of interest.
I would like to provide links to MEME institute and RBPMAP (you must provide your own motif for the latter).
http://meme-suite.org
http://rbpmap.technion.ac.il
you will need BED6 files to extract FASTA sequences.
Good luck
Hello,
miRNAs are very tricky- multiple miRNAs can affect a single gene and at the same time, single miRNA can affect many genes. Always try and identify the location of the gene. If it is in the 3' UTR region, the chances of miRNA having an effect on the gene expression is pretty high. We normally identify the target gene/regions and see the effect of any miRNA on the gene function, that way, it is easier to prove your point.
Good luck.
Venk
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