miRNA expression vector

Yijun Zhang @Yijun_Zhang

05 May 2010 1 377 Report

which vector is best to express a pre-miRNA? Can I buy it in addgen?

Yijun Zhang

Thanks for your suggestion , I will try. I am now trying a regular vector- pEGFP-c1, I hope it can work.

Badges
Science topic

More Yijun Zhang's questions See All

What is the best lysis buffer for his-tagged protein purification from 293T cells?

I want to purify a 6XHis-tagged protein from 293T cells, what lysis buffer should I use? After the cell lysis I will use Nickel Resin to purify this protein. Thanks

31 December 2014 9,639 4 View

Why don't my plasmids express the protein after several amplification?

We have constructed several plasmids that express HA- tagged proteins. At first, all the protein expression of these plasmids were confirmed by WB. But now, after several rounds of amplification...

09 October 2012 6,916 6 View

Which DNA/RNA/LNA synthesizer do you recommend?

We want to buy a DNA/RNA synthesizer, which can produce DNA primer, single strand and double strand RNA oligos with various modifications. I don't know which model is easy to use and reliable, are...

09 October 2012 6,607 5 View

What is a good selection to assay the expression of closely related microRNAs, such as members of let-7 family?

Taqman or LNA, or any other methods? If I chose one of these methods, should I also buy their related products, like RT kit and Real-time PCR assay kit, too?

02 March 2012 6,967 10 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

Does anybody face problems with total RNA isolated after miRNA mimic and inhibitor transfection?

Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...

13 May 2024 4,935 7 View

I would like to work on microRNA detect colorectal cancer. Which type of microRNA type micRNA124,micro143 how I can make primer design for it ?

would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?

07 May 2024 8,812 2 View