Yijun Zhang

5 Questions 24 Answers 0 Followers

Questions related from Yijun Zhang

What is the best lysis buffer for his-tagged protein purification from 293T cells?

I want to purify a 6XHis-tagged protein from 293T cells, what lysis buffer should I use? After the cell lysis I will use Nickel Resin to purify this protein. Thanks

01 January 2015 9,604 4 View

Why don't my plasmids express the protein after several amplification?

We have constructed several plasmids that express HA- tagged proteins. At first, all the protein expression of these plasmids were confirmed by WB. But now, after several rounds of amplification...

10 October 2012 6,873 6 View

Which DNA/RNA/LNA synthesizer do you recommend?

We want to buy a DNA/RNA synthesizer, which can produce DNA primer, single strand and double strand RNA oligos with various modifications. I don't know which model is easy to use and reliable, are...

10 October 2012 6,574 5 View

What is a good selection to assay the expression of closely related microRNAs, such as members of let-7 family?

Taqman or LNA, or any other methods? If I chose one of these methods, should I also buy their related products, like RT kit and Real-time PCR assay kit, too?

03 March 2012 6,934 10 View

miRNA expression vector

which vector is best to express a pre-miRNA? Can I buy it in addgen?

05 May 2010 335 1 View