Minimum delay beetween 2 sucrose preference tests in rat ?

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How can I calculate the logP of hyaluronic acid or other complex molecules?

I have been previously using https://www.molinspiration.com/services/logp.html to calculate log P for materials I use in my drug delivery research where it makes calculations based on canonical...

27 March 2024 9,780 0 View

How can I determine the appropriate dialysis pore size for my metal nanoparticles?

I am looking to preform a release study on iron oxide particles from a drug carrier, we have always used dialysis devices for other types of drug studies but I am slightly worried about the size...

18 February 2024 5,393 4 View

How to isolate interstitial and alveolar macrophages from lung for Western blot and Immunostaining ?

Hello everyone, I want to perform western blotting and immunofluorescence on macrophages isolated from mouse lung. I tried cell sorting in FACS with negative staining but I did not harvested...

11 December 2023 3,263 0 View

How can I get MRI phantom images of nanoparticles that are prone to aggregate/settle?

I am working with the polymeric modification of iron oxide particles and I am trying to get phantom MRI images at each stage of modification to comment on how the contrast effects are altered by...

26 November 2023 9,822 2 View

Is there a way to set specific temperature ramping rates on an Ohaus 7000 Guardian?

I am trying to synthesize Iron oxide nanoparticles via thermal decomposition and have read that temperature ramping rate plays a significant role in the reaction -- we did not have these...

18 October 2023 9,617 0 View

How can relaxivity be calculated from image intensity of an MRI phantom image?

I took MRI phantom images at various concentrations of iron (100, 50, 25, and 12.5 ug/mL) for my iron oxide based contrast agent system. The images came out as expected, with more darkening...

27 July 2023 2,025 3 View

What surfactants are the best for preventing aggregation in metal nanoparticles?

I have thus far been unable to get DLS characterizations of some ultrasmall iron oxide nanoparticles that I purchased commercially and I believe it is due to high rates of aggregration, especially...

10 June 2023 1,893 4 View

How can I get relaxivity measurements from a standard large animal MRI machine?

I am looking to characterize a contrast agent I'm developing for relaxivity. At my university, I have access to a Esaote Vet-MR Grande. Is it possible to get relaxation time measurements from this...

31 May 2023 3,807 3 View

How can I get accurate DLS measurements on my amine functionalized iron oxide nanoparticles?

I ordered 3nm iron oxide particles, amine functionalized, commercially. I am trying to use DLS to measure hydrodynamic diameter to later confirm additional coatings and functionalizations. I have...

25 April 2023 585 6 View

formula for calculating the spin orbit coupling of atoms with Z atomic number?

Is it possible to use this formula $E = En{n}*(Z\alpha)^2*(\frac{1}{j+1/2}-\frac{3}{4n})$ to determine the spin orbit coupling of an atom with atomic number Z at energy level n ? $\alpha=1/137$ is...

07 September 2020 5,974 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What is the anti anaemic potentials of hibiscus sabdariffa on wister rats?

anti anaemic potentials if hibiscus sabdariffa

01 July 2024 8,429 0 View

What is the best method to measure diameter of new vessels in tissue,e.g. spinal cord?

Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...

01 July 2024 9,744 0 View

Which pH range is allowed for subcutaneous pump administration?

Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...

19 June 2024 5,950 1 View