M. Pereira

3 Questions 5 Answers 0 Followers

Questions related from M. Pereira

Does anyone have a good protocol for bacterial extraction from endometrial samples?

Hello, I am trying to study bacterial composition in samples from endometrium. And to do so, we need to establish a protocol in the lab. I saw several papers, some used beads and mention which...

27 March 2019 6,913 3 View

Disruption issue: Chromatin immunoprecipitation in mouse brain tissue. Does anyone have a good sonication protocol?

Hello, I am trying to run ChiP with mouse brain tissue using active motif kit. I tried twice to run the optimization protocol for shearing conditions but I don't get any band in the agarose gel. I...

28 May 2018 8,290 3 View

How to dissolve starch for OSTT test?

Can anyone provide me with a good way to dissolve the starch when doing OSTT? I am running into problems to dissolve it since it gets kind of a jelly consistency after heating up the solution and...

11 January 2018 7,425 7 View