Hello, looking for TrpV1 knockout extracts from rat or human cells anyone have any?

Hello, I am testing some TrpV1 antibodies and would like to use KO extracts. I can find some whole mice but they are a bit steep for just some westerns. Does anyone have some KO extracts from...

09 October 2019 9,798 1 View

Can anyone recommend a good ELISA for COL2A1 (human)?

I have a small amount of money and time left on my project and would like to do an ELISA. I'm struggling to find one with any reviews and none of my colleagues can recommend one.

03 December 2018 2,508 2 View

Why does the mean droplet size of an emulsion changes with increasing the concentration of solid particles?

I am evaluating the effect of nanoparticle on emulsions. It was observed that when the increasing the concentration of the nanoparticles changes the mean droplet size (morphology). What is the...

24 July 2018 5,502 13 View

Minimum delay beetween 2 sucrose preference tests in rat ?

What is the minimum delay beetween 2 sucrose preference tests that one can perform for the rat (from one to 7 months) ?

16 January 2018 3,910 2 View

Neural network outputting average values of all outputs - Am I doing anything wrong?

14 January 2016 6,803 8 View

I am looking to compile a citation reference list for RNA-Seq; has anyone started one? Would anyone like to collaborate?

Hello, I would like to compile a citation reference list covering human RNA-Seq needs for researchers. I am wondering if anyone has already compiled a "guide line" list for the sequencing depth...

10 January 2016 1,370 2 View

How do I find degenerate PCR primers?

31 August 2015 3,824 3 View

What are the best non-metabolism based proliferation assays for cancer cells?

I have previously used CFSE for measuring the proliferation of lymphocytes. I now want to look at the proliferation of solid malignancy cancer cell lines, but I have read that CFSE may not be...

04 June 2014 3,545 5 View

Would another 50-ish students help?

We can probably add this to our preservice course. [email protected]

01 January 1970 364 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View