*Hypothesis: Particulate matter exposure will increase HIF-1alpha expression
*Cell: BEAS-2B cell
*Particulate matter: Sigma-Aldreich, PM10, commercial use
*Method of detection: Western Assay
BEAS-2B cell line (bronchial epithelial cell line) was exposed to PM10 in different conditions
#1. When exposed in 50mg/mL (6hr, 12 hr, 18 hr)
HIF-1 alpha was detected, and intensity was proportional to exposure time
#2. When exposed in 100mg/mL (6hr, 12 hr, 18 hr)
HIF-1 alpha was not detected (or minimally detected) in all conditions
*Cell viability was also assessed separately. As the PM density increased, cell viability was significantly reduced (proportionaly).
I was expecting strong expression in 100mg/mL condition.
What would be the reason for the minimal expression?
-Could decreasing cell viability have affected the results?
-Would MG132 10 mM use solve the problem?
-Strong ROS from higher density PM exposure could have affected the results? (We have
not performed regarding tests)
Any opinion is welcome! It could be a lot of help
Thank you very much!.
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